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当前位置:首页 > 抗原抗体、ELISA、WB > 一抗 > Polyclonal Antibodies > Vimentin Antibody

Vimentin Antibody

货号 货期 规格 / 价格 询价
APR06967G 100 μl / ¥4950

Vimentin Antibody

品牌

Leading Biology

货号

APR06967G

产品分类

Polyclonal Antibodies

研究领域

产品概述

We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality Vimentin Antibody.

分子量

53652 Da

细胞定位

Antigen Cellular Localization: Cytoplasm.

宿主

Rabbit

种属反应性

Human

靶点

This Vimentin antibody is generated from rabbits immunized with human Vimentin recombinant protein.

克隆号

RB41034

亚型

Rabbit Ig

基因ID

UniProt ID

功能

Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally.

总结

This gene encodes a member of the intermediate filamentfamily. Intermediate filamentents, along with microtubules andactin microfilaments, make up the cytoskeleton. The protein encodedby this gene is responsible for maintaining cell shape, integrityof the cytoplasm, and stabilizing cytoskeletal interactions. It isalso involved in the immune response, and controls the transport oflow-density lipoprotein (LDL)-derived cholesterol from a lysosometo the site of esterification. It functions as an organizer of anumber of critical proteins involved in attachment, migration, andcell signaling. Mutations in this gene causes a dominant,pulverulent cataract.

储存条件

Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

应用

WB, IHC-P, FC, IF, E

稀释方法

WB~~1:2000 IHC-P~~1:50 FC~~1:50 IF~~1:50

图像

VIME Antibody (Cat. APR06967G) western blot analysis in Hela,U251,A549,MDA-MB231 cell line lysates (35ug/lane).This demonstrates the VIME antibody detected the VIME protein (arrow).

Vimentin Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human small intestine tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of Vimentin Antibody for immunohistochemistry. Clinical relevance has not been evaluated.

Vimentin Antibody flow cytometric analysis of A549 cells (right histogram) compared to a negative control cell (left histogram).Alexa Fluor 488-conjugated donkey anti-rabbit lgG secondary antibodies were used for the analysis.

说明书

数量

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