品牌 |
Leading Biology | 货号 |
APR06115G |
产品分类 |
Polyclonal Antibodies | 研究领域 |
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产品概述 |
We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format.
We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team.
This product is a high quality VIME Antibody.
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分子量 |
53652 Da
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细胞定位 |
Antigen Cellular Localization:
Cytoplasm.
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宿主 |
Rabbit
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种属反应性 |
Human
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靶点 |
This Vimentin antibody is generated from rabbits immunized with Vimentin recombinant protein.
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亚型 |
Rabbit Ig
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基因ID |
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UniProt ID |
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功能 |
Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally.
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总结 |
VIME is a member of the intermediate filament family. Intermediate filamentents, along with microtubules and actin microfilaments, make up the cytoskeleton. This protein is responsible for maintaining cell shape, integrity of the cytoplasm, and stabilizing cytoskeletal interactions. It is also involved in the immune response, and controls the transport of low-density lipoprotein (LDL)-derived cholesterol from a lysosome to the site of esterification. It functions as an organizer of a number of critical proteins involved in attachment, migration, and cell signaling.
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储存条件 |
Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
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应用 |
WB, IHC-P, FC, E
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稀释方法 |
WB~~1:1000
IHC-P~~1:10~50
FC~~1:10~50
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图像 |
Western blot analysis of VIME Antibody (Cat. APR06115G) in A375 cell line lysates (35ug/lane). VIME (arrow) was detected using the purified Pab.
VIME Antibody (Cat. APR06115G)immunohistochemistry analysis in formalin fixed and paraffin embedded human rectum tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of VIME Antibody for immunohistochemistry. Clinical relevance has not been evaluated.
VIME Antibody (Cat. APR06115G) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis. |
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说明书 |
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数量 |
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