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CCNE1 Antibody (C-term)

APR05733G Product Datasheet Protocol

货号	货期	规格 / 价格	询价
APR05733G		100 μl / ￥4950

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安诺伦（北京）生物科技有限公司

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CCNE1 Antibody (C-term)

品牌	Leading Biology	货号	APR05733G
产品分类	Polyclonal Antibodies	研究领域
产品概述	We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality CCNE1 Antibody (C-term).
分子量	47077 Da
细胞定位	Antigen Cellular Localization: Nucleus.
宿主	Rabbit
种属反应性	Human
免疫原	373-402 aa
靶点	This CCNE1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 373-402 amino acids from the C-terminal region of human CCNE1.
亚型	Rabbit Ig
通用名	CCNE
基因ID	898
UniProt ID	P24864
功能	Essential for the control of the cell cycle at the G1/S (start) transition.
总结	Cyclin E1 belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. Cyclin E1 forms a complex with and functions as a regulatory subunit of CDK2, whose activity is required for cell cycle G1/S transition. Cyclin E1 accumulates at the G1-S phase boundary and is degraded as cells progress through S phase. Overexpression of Cyclin E1 has been observed in many tumors, which results in chromosome instability, and thus may contribute to tumorigenesis. This protein was found to associate with, and be involved in, the phosphorylation of NPAT protein (nuclear protein mapped to the ATM locus), which participates in cell-cycle regulated histone gene expression and plays a critical role in promoting cell-cycle progression in the absence of pRB.
储存条件	Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
应用	WB, IHC-P, FC, E
稀释方法	WB~~1:1000 IHC-P~~1:50~100 FC~~1:10~50
图像	Anti-Cyclin E1 Antibody at 1:1000 dilution + A2058 whole cell lysate Lysates/proteins at 20 μg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 47 kDa Blocking/Dilution buffer: 5% NFDM/TBST. Formalin-fixed and paraffin-embedded human testis tissue with Cyclin E1 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. Flow cytometric analysis of NCI-H292 cells using Cyclin E1 Antibody (C-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
说明书	Product Datasheet Protocol
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