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RSPO2 Antibody (C-term)

APR05604G Product Datasheet Protocol

货号	货期	规格 / 价格	询价
APR05604G		100 μl / ￥4950

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RSPO2 Antibody (C-term)

品牌	Leading Biology	货号	APR05604G
产品分类	Polyclonal Antibodies	研究领域
产品概述	We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality RSPO2 Antibody (C-term).
分子量	28315 Da
细胞定位	Antigen Cellular Localization: Secreted.
宿主	Rabbit
种属反应性	Human
免疫原	190-218 aa
靶点	This RSPO2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 190-218 amino acids from the C-terminal region of human RSPO2.
亚型	Rabbit Ig
基因ID	340419
UniProt ID	Q6UXX9
功能	Activator of the canonical Wnt signaling pathway by acting as a ligand for LGR4-6 receptors. Upon binding to LGR4-6 (LGR4, LGR5 or LGR6), LGR4-6 associate with phosphorylated LRP6 and frizzled receptors that are activated by extracellular Wnt receptors, triggering the canonical Wnt signaling pathway to increase expression of target genes. Also regulates the canonical Wnt/beta-catenin-dependent pathway and non-canonical Wnt signaling by acting as an inhibitor of ZNRF3, an important regulator of the Wnt signaling pathway. Probably also acts as a ligand for frizzled and LRP receptors.
总结	R-spondins (RSPOs), such as RSPO2, are secreted proteins that regulate beta-catenin (CTNNB1; MIM 116806) signaling.
储存条件	Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
应用	IHC-P, FC, WB, E
稀释方法	WB~~1:1000 IHC-P~~1:50~100 FC~~1:10~50
图像	Western blot analysis of lysate from 293 cell line, using RSPO2 Antibody (C-term)(Cat. APR05604G). APR05604G was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug per lane. RSPO2 Antibody (C-term) (Cat. APR05604G) immunohistochemistry analysis in formalin fixed and paraffin embedded human lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the RSPO2 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated. RSPO2 Antibody (C-term) (Cat. APR05604G) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
说明书	Product Datasheet Protocol
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