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当前位置:首页 > 抗原抗体、ELISA、WB > 一抗 > Polyclonal Antibodies > UBE4A Antibody (C-term)

UBE4A Antibody (C-term)

货号 货期 规格 / 价格 询价
APR04779G 100 μl / ¥4950

UBE4A Antibody (C-term)

品牌

Leading Biology

货号

APR04779G

产品分类

Polyclonal Antibodies

研究领域

产品概述

We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality UBE4A Antibody (C-term).

分子量

122561 Da

细胞定位

Antigen Cellular Localization: Cytoplasm {ECO:0000250|UniProtKB:E9Q735}.

宿主

Rabbit

种属反应性

Human

免疫原

1037-1067 aa

靶点

This UBE4A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1037-1067 amino acids from the C-terminal region of human UBE4A.

亚型

Rabbit Ig

基因ID

UniProt ID

功能

Ubiquitin-protein ligase that probably functions as an E3 ligase in conjunction with specific E1 and E2 ligases. May also function as an E4 ligase mediating the assembly of polyubiquitin chains on substrates ubiquitinated by another E3 ubiquitin ligase. Mediates 'Lys-48'-linked polyubiquitination of substrates.

总结

Ubiquitination requires a ubiquitin-activating enzyme, a ubiquitin-conjugating enzyme, and usually a substrate-specific ubiquitin-protein ligase. Multiubiquitination needs a fourth factor, to bind to the ubiquitin moieties of preformed conjugates and catalyze ubiquitin chain assembly in conjunction with the first three proteins.1 Human protein KIAA0126, with a predicted 1,073 amino acid residues, is a member of the novel protein family defined by ubiquitination conjugation factor E4.2 In yeast, E4 activity is linked to cell survival under stress conditions, indicating that eucaryotes use E4-dependent proteolysis pathways for multiple cellular functions.

储存条件

Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

应用

WB, IHC-P, E

稀释方法

WB~~1:1000 IHC-P~~1:50~100

图像

Anti-UBE4A Antibody (T1052) at 1:1000 dilution + K562 whole cell lysate Lysates/proteins at 20 μg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 123 kDa Blocking/Dilution buffer: 5% NFDM/TBST.

Western blot analysis of anti-UBE4A Antibody (C-term) (Cat.APR04779G) in NCI-H460 and CEM cell line lysates (35ug/lane). UBE4A (arrow) was detected using the purified Pab.

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

说明书

数量

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