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KRAS Antibody (C-term)

APR04099G Product Datasheet Protocol

货号	货期	规格 / 价格	询价
APR04099G		100 μl / ￥4950

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KRAS Antibody (C-term)

品牌	Leading Biology	货号	APR04099G
产品分类	Polyclonal Antibodies	研究领域
产品概述	We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality KRAS Antibody (C-term).
分子量	21656 Da
细胞定位	Antigen Cellular Localization: Cell membrane; Lipid-anchor; Cytoplasmic side. Cytoplasm, cytosol
宿主	Rabbit
种属反应性	Human, Mouse, Rat
免疫原	146-174 aa
靶点	This KRAS antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 146-174 amino acids from the C-terminal region of human KRAS.
亚型	Rabbit Ig
通用名	KRAS2, RASK2
基因ID	3845
UniProt ID	P01116
功能	Ras proteins bind GDP/GTP and possess intrinsic GTPase activity. Plays an important role in the regulation of cell proliferation (PubMed:23698361, PubMed:22711838).
总结	This gene, a Kirsten ras oncogene homolog from themammalian ras gene family, encodes a protein that is a member ofthe small GTPase superfamily. A single amino acid substitution isresponsible for an activating mutation. The transforming proteinthat results is implicated in various malignancies, including lungadenocarcinoma, mucinous adenoma, ductal carcinoma of the pancreasand colorectal carcinoma. Alternative splicing leads to variantsencoding two isoforms that differ in the C-terminal region.
储存条件	Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
应用	WB, IF, FC, E
稀释方法	WB~~1:2000 IF~~1:10~50 FC~~1:10~50
图像	All lanes : Anti-KRAS Antibody (C-term) at 1:2000 dilution Lane 1: 293T/17 whole cell lysate Lane 2: C2C12 whole cell lysate Lane 3: Hela whole cell lysate Lane 4: HT-29 whole cell lysate Lane 5: K562 whole cell lysate Lane 5: PC-12 whole cell lysate Lane 5: Ramos whole cell lysate Lysates/proteins at 20 μg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 22 kDa Blocking/Dilution buffer: 5% NFDM/TBST. Confocal immunofluorescent analysis of KRAS Antibody (C-term)( APR04099G) with WiDr cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue). KRAS Antibody (C-term) (Cat. APR04099G) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
说明书	Product Datasheet Protocol
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