品牌 |
Leading Biology | 货号 |
APR03994G |
产品分类 |
Polyclonal Antibodies | 研究领域 |
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产品概述 |
We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format.
We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team.
This product is a high quality UBE4A Antibody (Center).
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分子量 |
122561 Da
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细胞定位 |
Antigen Cellular Localization:
Cytoplasm {ECO:0000250|UniProtKB:E9Q735}.
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宿主 |
Rabbit
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种属反应性 |
Human
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免疫原 |
451-479 aa
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靶点 |
This UBE4A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 451-479 amino acids from the Central region of human UBE4A.
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亚型 |
Rabbit Ig
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基因ID |
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UniProt ID |
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功能 |
Ubiquitin-protein ligase that probably functions as an E3 ligase in conjunction with specific E1 and E2 ligases. May also function as an E4 ligase mediating the assembly of polyubiquitin chains on substrates ubiquitinated by another E3 ubiquitin ligase. Mediates 'Lys-48'-linked polyubiquitination of substrates.
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总结 |
The modification of proteins with ubiquitin is animportant cellular mechanism for targeting abnormal or short-livedproteins for degradation. Ubiquitination involves at least threeclasses of enzymes: ubiquitin-activating enzymes, or E1s,ubiquitin-conjugating enzymes, or E2s, and ubiquitin-proteinligases, or E3s. This gene encodes an additional conjugationfactor, E4, which is involved in multiubiquitin chain assembly.
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储存条件 |
Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
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应用 |
WB, IHC-P, E
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稀释方法 |
WB~~1:1000
IHC-P~~1:10~50
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图像 |
All lanes : Anti-UBE4A Antibody (Center) at 1:1000 dilution Lane 1: Caco2 whole cell lysate Lane 2: HepG2 whole cell lysate Lysates/proteins at 20 μg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 123 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
UBE4A Antibody (Center) (Cat. APR03994G) western blot analysis in ZR-75-1 cell line lysates (35ug/lane).This demonstrates the UBE4A antibody detected the UBE4A protein (arrow).
UBE4A Antibody (Center) (APR03994G)immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of UBE4A Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated. |
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说明书 |
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数量 |
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