品牌 |
Leading Biology | 货号 |
APR03986G |
产品分类 |
Polyclonal Antibodies | 研究领域 |
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产品概述 |
We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format.
We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team.
This product is a high quality CNOT4 Antibody.
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分子量 |
63510 Da
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细胞定位 |
Antigen Cellular Localization:
Cytoplasm. Nucleus
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宿主 |
Rabbit
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种属反应性 |
Human
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靶点 |
This CNOT4 antibody is generated from rabbits immunized with human CNOT4 recombinant protein.
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亚型 |
Rabbit Ig
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通用名 |
NOT4
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基因ID |
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UniProt ID |
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功能 |
Has E3 ubiquitin ligase activity. Involved in activation of the JAK/STAT pathway.
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总结 |
CNOT4 has intrinsic E3 ubiquitin ligase activity. Binding of the CNOT4 RING finger to the ubiquitin-conjugating enzyme (E2) UbcH5B is highly selective.The CCR4-NOT complex functions as a global regulator of RNA polymerase II transcription.
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储存条件 |
Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
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应用 |
WB, IHC-P, FC, E
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稀释方法 |
WB~~1:1000
IHC-P~~1:50~100
FC~~1:10~50
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图像 |
Western blot analysis of CNOT4 Antibody (Cat. APR03986G) in MCF-7, K562, 293 cell line lysates (35ug/lane). CNOT4 (arrow) was detected using the purified Pab.
Formalin-fixed and paraffin-embedded human testis tissue reacted with CNOT4 Antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
CNOT4 Antibody (Cat. APR03986G) flow cytometry analysis of MCF-7 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis. |
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说明书 |
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数量 |
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