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OPN-a/b Antibody (N-term)

APR03579G Product Datasheet Protocol

货号	货期	规格 / 价格	询价
APR03579G		100 μl / ￥4950

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OPN-a/b Antibody (N-term)

品牌	Leading Biology	货号	APR03579G
产品分类	Polyclonal Antibodies	研究领域
产品概述	We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality OPN-a/b Antibody (N-term).
分子量	35423 Da
细胞定位	Antigen Cellular Localization: Secreted.
宿主	Rabbit
种属反应性	Human
免疫原	14-40 aa
靶点	This OPN-a/b antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 14-40 amino acids from the N-terminal region of human OPN-a/b.
亚型	Rabbit Ig
通用名	BNSP, OPN
基因ID	6696
UniProt ID	P10451
功能	Binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction.
总结	Binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction.Acts as a cytokine involved in enhancing production of interferon-gamma and interleukin-12 and reducing production of interleukin-10 and is essential in the pathway that leads to type I immunity (By similarity).
储存条件	Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
应用	WB, IF, IHC-P, E
稀释方法	WB~~1:1000 IF~~1:25 IHC-P~~1:50~100
图像	All lanes : Anti-OPN-a/b Antibody (N-term) at 1:2000 dilution Lane 1: Hela whole cell lysate Lane 2: HL-60 whole cell lysate Lane 3: Jurkat whole cell lysate Lane 4: MOLT-4 whole cell lysate Lysates/proteins at 20 μg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 35 kDa Blocking/Dilution buffer: 5% NFDM/TBST. Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0. 1% Triton X-100 permeabilized MCF-7 (human breast cancer cell line) cells labeling Pdx1 with APR03579G at 1/25 dilution, followed by Dylight? 488-conjugated goat anti-rabbit IgG (NK179883) secondary antibody at 1/200 dilution (green). Immunofluorescence image showing cytoplasm staining on MCF-7 cell line. Cytoplasmic actin is detected with Dylight? 554 Phalloidin (PD18466410) at 1/100 dilution (red). The nuclear counter stain is DAPI (blue). Anti-OPN-a/b Antibody (N-term) at 1:2000 dilution + Hela whole cell lysate Lysates/proteins at 20 μg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 35 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
说明书	Product Datasheet Protocol
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