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S100A6 Calcyclin

货号 货期 规格 / 价格 询价

S100A6 Calcyclin

品牌

Swant

货号

pCAcy

产品分类

Recombinant Protein

研究领域

Others

产品概述

Reconstitute with 10 ul bidistilled water. Freeze in small aliquots (e.g. 1 or 10 ug) and keep at -80°C. Avoid repeated freezing and thawing.

形式

This protein has been purified from mouse Ehrlich ascites cells by HPLC (see Kuznicki et al. (1989) Biochem. J. 263: 951-956). This protein can be used for immunoblots, absorption experiments in immunohistochemistry, radioimmunoassay and intracellular injection. For fluid-phase adsorption in immunohistochemistry we suggest the following procedure: - Dilute 1 µl of the antiserum against S100A6 (calcyclin) (Swant) in 5 ml of the usual buffer for immunohistochemistry (final dilution 1:5'000). - Add 1 µg of the purified protein to 1 ml of the diluted antibody solution and mix well. - Incubate for at least 6 hours in the cold. - Apply to tissue-sections and incubate for 3 days. - Complete the immunohistochemical reaction as usual (biotinylated second antibody, ABC-complex, DAB). As a result, the immunostaining should be strongly reduced or even completely prevented.

浓度

S100A6 (calcyclin) antigen (10 ug)

纯度

Mouse

试剂准备

lyophilized in ddH2O

产品描述

Purification: Chromatogr. Lot No: 2.’96

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