品牌 |
Swant | 货号 |
AgPV10abs |
产品分类 |
Recombinant Protein | 研究领域 |
Parvalbumin |
产品概述 |
After reconstitution freeze in small aliquots (e.g. 1 ug) and keep at -80°C. Avoid repeated freezing and thawing.
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形式 |
This recombinant protein has been purified by HPLC and migrates as a single band of approximately 12 kDa. The figure shows a gel stained with Coomassie-blue.
For adsorption experiments aimed at proofing the specificity of the immunostaining, we suggest the following
procedure:
- Dilute 1 µl of one of the Swants antibodies against parvalbumin in 5 ml of the usual buffer for
immunohistochemistry (final dilution 1:5'000).
- Add 1 µg of the recombinant protein to 1 ml of the diluted antibody solution and mix well.
- Incubate for at least 6 hours in the cold.
- Apply to tissue-sections and incubate for 3 days.
- Complete the immunohistochemical reaction as usual (biotinylated secondary antibody, Streptavidin-Cy2 (or
other fluorescent dyes), or peroxidase (followed by DAB-H2O2).
As a result, the immunostaining should be strongly reduced or even completely prevented
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浓度 |
HPLC-purified mouse parvalbumin (recombinant, 10 g) produced in Escherichia Coli, after in vitro cDNA synthesis. |
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纯度 |
Recombinant mouse |
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试剂准备 |
10 ug in 25 ul, 20 mM NaHCo3 with high CaCl2 (0.1 mM). Lyophilized.
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应用 |
This protein can be used for immunoblots and absorption experiments in immunehistochemistry. For intracellular injections in neurons, we suggest using our other product AgPV10ic or AgPV100ic.
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产品描述 |
Purification: HPLC
Lot No: 11 (20-17)
Reconstitution: with 25 ul of bi-distilled water.
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官网链接 |
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数量 |
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选择 | 品牌 | 货号 | 产品名称 | 规格 | 分类 | 研究领域 | 说明书 | 数量 | 目录价 | |
1 | Swant | rCB9 | Calbindin D-9k | 60 µg | Recombinant Protein | Calbindin |
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¥8488.00 | 订购 询价 | |
2 | Swant | AgPV100ic | Parvalbumin | 100 µg | Recombinant Protein | Parvalbumin |
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¥8138.00 | 订购 询价 | |
3 | Swant | rCB28 | Calbindin D-28k | 10 µg | Recombinant Protein | Calbindin |
|
¥3850.00 | 订购 询价 | |
4 | Swant | rCRh100 | Calretinin | 100 µg | Recombinant Protein | Calretinin |
|
¥12688.00 | 订购 询价 | |
5 | Swant | AgPV10ic | Parvalbumin | 10 µg | Recombinant Protein | Parvalbumin |
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¥3150.00 | 订购 询价 | |
6 | Swant | AgCB10ic | Calbindin D-28k | 10 µg | Recombinant Protein | Calbindin |
|
¥3150.00 | 订购 询价 |
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