品牌 |
Swant | 货号 |
rCRh100 |
产品分类 |
Recombinant Protein | 研究领域 |
Calretinin |
产品概述 |
Freeze in small aliquots (e.g. 1 or 10 ug) and keep at -80°C. Avoid repeated freezing and thawing.
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形式 |
This recombinant protein has been purified by affinity chromatography on a NTA-resin
This protein can be used for immunoblots, absorption experiments in immunohistochemistry, radioimmunoassay and intracellular injection.
For adsorption we suggest the following procedure:
- Dilute 1 ul of the antiserum 7696 (SWant) against calretinin in 5 ml of the usual buffer for immunohistochemistry (final dilution 1:5'000). - Add 1 ug of the recombinant protein to 1 ml of the diluted antibody solution and mix well.
- Incubate for at least 6 hours in the cold.
- Apply to tissue-sections and incubate for 3 days.
- Complete the immunohistochemical reaction as usual (biotinylated second antibody, ABC-complex, DAB).
As a result, the immunostaining should be strongly reduced or even completely prevented.
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浓度 |
6-His human calretinin (recombinant) produced in Escherichia Coli. |
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纯度 |
Recombinant human |
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试剂准备 |
lyophilized (10 or 100 ug in 25, resp. 155 ul of: 10 mM Tris, 50 mM NaCl, 2 mM CaCl2, 1 mM EDTA, 1mM 脽-mercaptoethanol, pH 7.4).
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复溶 |
Schwaller B., Buchwald P., Blümcke I., Celio M.R. and Hunziker W. (1993) Characterization of a polyclonal antiserum against the purified human recombinant calcium-binding protein calretinin. Cell Calcium 14: 639-648.
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产品描述 |
Purification: Affinity chromat.
Lot No: 22
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官网链接 |
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数量 |
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选择 | 品牌 | 货号 | 产品名称 | 规格 | 分类 | 研究领域 | 说明书 | 数量 | 目录价 | |
1 | Swant | rCB9 | Calbindin D-9k | 60 µg | Recombinant Protein | Calbindin |
|
¥8488.00 | 订购 询价 | |
2 | Swant | AgPV100ic | Parvalbumin | 100 µg | Recombinant Protein | Parvalbumin |
|
¥8138.00 | 订购 询价 | |
3 | Swant | AgPV10abs | Parvalbumin | 10 µg | Recombinant Protein | Parvalbumin |
|
¥3150.00 | 订购 询价 | |
4 | Swant | rCB28 | Calbindin D-28k | 10 µg | Recombinant Protein | Calbindin |
|
¥3850.00 | 订购 询价 | |
5 | Swant | AgPV10ic | Parvalbumin | 10 µg | Recombinant Protein | Parvalbumin |
|
¥3150.00 | 订购 询价 | |
6 | Swant | AgCB10ic | Calbindin D-28k | 10 µg | Recombinant Protein | Calbindin |
|
¥3150.00 | 订购 询价 |
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