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QuickTiter? MuLV Core Antigen ELISA Kit

VPK-156 SDS Protocol

货号	货期	规格 / 价格	询价
VPK-156		96 assays / ￥12121

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QuickTiter? MuLV Core Antigen ELISA Kit

品牌	Cell Biolabs	货号	VPK-156
产品分类	ELISA试剂盒	研究领域	Cell biology
产品概述	An anti-MuLV p30 monoclonal coating antibody is adsorbed onto a microtiter plate. MuLV core antigen (p30) present in the sample or standard binds to the antibodies adsorbed on the plate; an anti-MuLV p30 polyclonal antibody is added and binds to the antigen captured by the first antibody. Following incubation and wash steps, a HRP-conjugated secondary antibody is added and binds to the anti-MuLV p30 polyclonal. Unbound HRP-conjugated secondary antibody is removed during the wash steps, and substrate solution reactive with HRP is added to the wells. A colored product is formed in proportion to the amount of MuLV core antigen present in the sample. The reaction is terminated by addition of acid and absorbance is measured at 450 nm. A standard curve is prepared from recombinant MuLV p30 core antigen and sample concentration is then determined.
储存条件	Upon receipt, aliquot and store the Recombinant MuLV p30 Standard at -20oC to avoid multiple freeze/thaw cycles. Store all other kit components at 4oC.
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上一产品：ViraSafe? Lentiviral Packaging System, Ecotropic 下一产品：Calbindin D-28k Sandwich-ELISA

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