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CytoSelect? BrdU Cell Proliferation ELISA Kit

CBA-251 SDS Protocol

货号	货期	规格 / 价格	询价
CBA-251		96 assays / ￥6563

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CytoSelect? BrdU Cell Proliferation ELISA Kit

品牌	Cell Biolabs	货号	CBA-251
产品分类	ELISA试剂盒	研究领域	Cell biology
产品概述	The CytoSelect? BrdU Cell Proliferation ELISA Kit detects BrdU incorporated into cellular DNA during cell proliferation using an anti-BrdU antibody. ?When cells are incubated in media containing BrdU, the pyrimidine analog is incorporated in place of thymidine into the newly synthesized DNA of proliferating cells. ?Once the labeling media is removed, the cells are fixed and the DNA is denatured in one step with a fix/denature solution (denaturation of the DNA is necessary to improve the accessibility of the incorporated BrdU for detection). ?Then an anti-BrdU mouse monoclonal antibody is added followed by an HRP conjugated secondary antibody to detect the incorporated BrdU. ?The magnitude of the absorbance for the developed color is proportional to the quantity of BrdU incorporated into cells and can be directly correlated to cell proliferation.
储存条件	Upon receipt, store the 1000X BrdU Solution and Anti-BrdU Monoclonal Antibody at -20oC. Store all other components at 4oC.
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上一产品：OxiSelect? TBARS Assay Kit (MDA Quantitation) 下一产品：Calbindin D-28k Sandwich-ELISA

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