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UBB Antibody

APR10629G Product Datasheet Protocol

货号	货期	规格 / 价格	询价
APR10629G		100 μl / ￥5450

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UBB Antibody

品牌	Leading Biology	货号	APR10629G
产品分类	Monoclonal Antibodies	研究领域
产品概述	We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality UBB antibody.
分子量	26kDa
细胞定位	Antigen Cellular Localization: Ubiquitin: Cytoplasm. Nucleus
宿主	Mouse
种属反应性	Human
克隆号	3C12
亚型	IgG1
基因ID	7314
UniProt ID	P0CG47
功能	Ubiquitin: Exists either covalently attached to another protein, or free (unanchored). When covalently bound, it is conjugated to target proteins via an isopeptide bond either as a monomer (monoubiquitin), a polymer linked via different Lys residues of the ubiquitin (polyubiquitin chains) or a linear polymer linked via the initiator Met of the ubiquitin (linear polyubiquitin chains). Polyubiquitin chains, when attached to a target protein, have different functions depending on the Lys residue of the ubiquitin that is linked: Lys-6-linked may be involved in DNA repair; Lys-11-linked is involved in ERAD (endoplasmic reticulum-associated degradation) and in cell-cycle regulation; Lys-29-linked is involved in lysosomal degradation; Lys-33-linked is involved in kinase modification; Lys-48-linked is involved in protein degradation via the proteasome; Lys-63-linked is involved in endocytosis, DNA-damage responses as well as in signaling processes leading to activation of the transcription factor NF-kappa-B. Linear polymer chains formed via attachment by the initiator Met lead to cell signaling. Ubiquitin is usually conjugated to Lys residues of target proteins, however, in rare cases, conjugation to Cys or Ser residues has been observed. When polyubiquitin is free (unanchored-polyubiquitin), it also has distinct roles, such as in activation of protein kinases, and in signaling.
形式	Liquid. In PBS containing 0.02% sodium azide.
储存条件	Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
应用	WB, FC, E
稀释方法	E~~1/10000 WB~~1/500 - 1/2000 FC~~1/200 - 1/400
别名	Polyubiquitin-B, Ubiquitin, UBB
图像	Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng); Figure 1: Western blot analysis using UBB mAb against human UBB (AA: 1-299) recombinant protein. (Expected MW is 26 kDa) Figure 2: Western blot analysis using UBB mouse mAb against NIH/3T3 (1) and Hela (2) cell lysate.
说明书	Product Datasheet Protocol
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