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PGK1

APR09086G Product Datasheet Protocol

货号	货期	规格 / 价格	询价
APR09086G		100 μl / ￥4950

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PGK1

品牌	Leading Biology	货号	APR09086G
产品分类	Monoclonal Antibodies	研究领域
产品概述	We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality PGK1.
分子量	44615 Da
细胞定位	Antigen Cellular Localization: Cytoplasm.
宿主	Mouse
种属反应性	Human, Mouse
靶点	This antibody is generated from a mouse immunized with a KLH conjugated synthetic peptide between 1-417 amino acids from human.
克隆号	1086CT10.2.1
亚型	IgG2a,k
通用名	PGKA
基因ID	5230
UniProt ID	P00558
功能	In addition to its role as a glycolytic enzyme, it seems that PGK-1 acts as a polymerase alpha cofactor protein (primer recognition protein).
总结	In addition to its role as a glycolytic enzyme, it seems that PGK-1 acts as a polymerase alpha cofactor protein (primer recognition protein).
形式	Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
储存条件	Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
应用	IHC-P, FC, WB, E
稀释方法	IHC-P~~1:25 FC~~1:25 WB~~1:8000
别名	Phosphoglycerate kinase 1, 2.7.2.3, Cell migration-inducing gene 10 protein, Primer recognition protein 2, PRP 2, PGK1, PGKA
图像	APR09086G staining PGK1 in human liver tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody. APR09086G staining PGK1 in human kidney tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody. Overlay histogram showing Jurkat cells stained with APR09086G(green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (APR09086G, 1:25 dilution) for 60 min at 37?C. The secondary antibody used was Goat-Anti-Mouse IgG, DyLight? 488 Conjugated Highly Cross-Adsorbed(OJ192088) at 1/200 dilution for 40 min at 37?C. Isotype control antibody (blue line) was mouse IgG2a (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
说明书	Product Datasheet Protocol
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