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RAB27A Antibody

AMM07444G Product Datasheet Protocol

货号	货期	规格 / 价格	询价
AMM07444G		100 μl / ￥4950

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RAB27A Antibody

品牌	Leading Biology	货号	AMM07444G
产品分类	Monoclonal Antibodies	研究领域
产品概述	We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality RAB27A antibody.
分子量	24868 Da
细胞定位	Antigen Cellular Localization: Membrane; Lipid-anchor. Melanosome. Late endosome. Lysosome. Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV. Localizes to endosomal exocytic vesicles
宿主	Mouse
种属反应性	Human
靶点	This RAB27A antibody is generated from a mouse immunized with a recombinant protein of human RAB27A.
克隆号	1590CT813.266.26
亚型	IgG1,k
通用名	RAB27
基因ID	5873
UniProt ID	P51159
功能	Plays a role in cytotoxic granule exocytosis in lymphocytes. Required for both granule maturation and granule docking and priming at the immunologic synapse.
总结	Plays a role in cytotoxic granule exocytosis in lymphocytes. Required for both granule maturation and granule docking and priming at the immunologic synapse.
形式	Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
储存条件	Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
应用	FC, WB, E
稀释方法	FC~~1:25 WB~~1:2000
别名	Ras-related protein Rab-27A, Rab-27, GTP-binding protein Ram, RAB27A, RAB27
图像	Overlay histogram showing K562 cells stained with AMM07444G(green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AMM07444G, 1:25 dilution) for 60 min at 37?C. The secondary antibody used was Goat-Anti-Mouse IgG, DyLight? 488 Conjugated Highly Cross-Adsorbed(OJ192088) at 1/200 dilution for 40 min at 37?C. Isotype control antibody (blue line) was mouse IgG1 (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed. Anti-RAB27A Antibody at 1:2000 dilution + K562 whole cell lysate Lysates/proteins at 20 μg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 25 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
说明书	Product Datasheet Protocol
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