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LAMP1 Antibody

AMM08783G Product Datasheet Protocol

货号	货期	规格 / 价格	询价
AMM08783G		100 μl / ￥4950

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安诺伦（北京）生物科技有限公司

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LAMP1 Antibody

品牌	Leading Biology	货号	AMM08783G
产品分类	Monoclonal Antibodies	研究领域
产品概述	We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality LAMP1 antibody.
分子量	44882 Da
细胞定位	Antigen Cellular Localization: Cell membrane; Single-pass type I membrane protein. Endosome membrane; Single-pass type I membrane protein Lysosome membrane; Single-pass type I membrane protein. Late endosome. Note=This protein shuttles between lysosomes, endosomes, and the plasma membrane. Colocalizes with OSBPL1A at the late endosome
宿主	Mouse
种属反应性	Human, African Green Monkey
靶点	This LAMP1 antibody is generated from a mouse immunized with a recombinant protein.
克隆号	1483CT517.45.23
亚型	IgG1,k
基因ID	3916
UniProt ID	P11279
功能	Presents carbohydrate ligands to selectins. Also implicated in tumor cell metastasis.
总结	Presents carbohydrate ligands to selectins. Also implicated in tumor cell metastasis.
形式	Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
储存条件	Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
应用	IF, IHC-P, WB, E
稀释方法	IF~~1:25 IHC-P~~1:25 WB~~1:2000
别名	Lysosome-associated membrane glycoprotein 1, LAMP-1, Lysosome-associated membrane protein 1, CD107 antigen-like family member A, CD107a, LAMP1
图像	Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0. 1% Triton X-100 permeabilized HeLa (human cervical epithelial adenocarcinoma cell line) cells labeling Pdx1 with AMM08783G at 1/25 dilution, followed by Dylight? 488-conjugated goat anti-mouse IgG (NA166821) secondary antibody at 1/200 dilution (green). Immunofluorescence image showing cytoplasm staining on HeLa cell line. Cytoplasmic actin is detected with Dylight? 554 Phalloidin (PD18466410) at 1/100 dilution (red). The nuclear counter stain is DAPI (blue). AMM08783G staining LAMP1 in human kidney sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody. All lanes : Anti-LAMP1 Antibody at 1:2000 dilution Lane 1: A431 whole cell lysates Lane 2: Hela whole cell lysates Lane 3: Jurkat whole cell lysates Lane 4: HT-1080 whole cell lysates Lane 5: COS-7 whole cell lysates Lysates/proteins at 20 μg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 45 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
说明书	Product Datasheet Protocol
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