品牌 |
Leading Biology | 货号 |
APR10198G |
产品分类 |
Monoclonal Antibodies | 研究领域 |
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产品概述 |
We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format.
We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team.
This product is a high quality SOD2 antibody.
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分子量 |
24722 Da
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细胞定位 |
Antigen Cellular Localization:
Mitochondrion matrix.
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宿主 |
Mouse
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种属反应性 |
Human
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靶点 |
Purified His-tagged SOD protein(Fragment) was used to produced this monoclonal antibody.
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克隆号 |
37CT127.5.11.6
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亚型 |
IgG1κ
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基因ID |
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UniProt ID |
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功能 |
Destroys superoxide anion radicals which are normally produced within the cells and which are toxic to biological systems.
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总结 |
This gene is a member of the iron/manganese superoxide dismutase family. It encodes a mitochondrial protein that forms a homotetramer and binds one manganese ion per subunit. This protein binds to the superoxide byproducts of oxidative phosphorylation and converts them to hydrogen peroxide and diatomic oxygen. Mutations in this gene have been associated with idiopathic cardiomyopathy (IDC), premature aging, sporadic motor neuron disease, and cancer. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.
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形式 |
Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
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储存条件 |
Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
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应用 |
IHC-P, WB, E
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稀释方法 |
WB~~1:2000
IHC-P~~1:10~50
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别名 |
Superoxide dismutase [Mn], mitochondrial, SOD2
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图像 |
All lanes : Anti-SD Antibody at 1:2000 dilution Lane 1: Hela whole cell lysates Lane 2: human brain lysates Lane 3: human heart lysates Lane 4: human liver lysates Lysates/proteins at 20 μg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 25 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with SOD2 Monoclonal Antibody (Cat. APR10198G), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Western blot analysis of anti-SOD2 Monoclonal Antibody (Cat. APR10198G) by SOD2-GST fusion protein (GST MW=26 kD. SOD2-GST fusion protein (arrow) was detected using the purified Mab. (1:2000) |
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说明书 |
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数量 |
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