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Beclin 1 Antibody (Ascites)

APR06985G Product Datasheet Protocol

货号	货期	规格 / 价格	询价
APR06985G		0.1 ml / ￥4950

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Beclin 1 Antibody (Ascites)

品牌	Leading Biology	货号	APR06985G
产品分类	Monoclonal Antibodies	研究领域
产品概述	We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality Beclin 1 antibody (Ascites).
分子量	51896 Da
细胞定位	Antigen Cellular Localization: Golgi apparatus, trans-Golgi network membrane; Peripheral membrane protein. Note=Interaction with ATG14 promotes translocation to autophagosomes. Expressed in dendrites and cell bodies of cerebellar Purkinje cells (By similarity)
宿主	Mouse
种属反应性	Human
靶点	This Beclin 1 antibody was raised using purified His-tagged recombinant full length human Autophagy BECN1.
克隆号	15CT26
亚型	IgG3λ
通用名	GT197
基因ID	8678
UniProt ID	Q14457
功能	Plays a central role in autophagy (PubMed:23184933). Required for the abcission step in cytokinesis (PubMed:20208530). Protects against infection by a neurovirulent strain of Sindbis virus (PubMed:9765397). May play a role in antiviral host defense.
总结	Beclin-1 participates in the regulation of autophagy and has an important role in development, tumorigenesis, and neurodegeneration (Zhong et al., 2009 [PubMed 19270693]).
形式	Mouse monoclonal antibody supplied in crude ascites with 0.09% (W/V) sodium azide.
储存条件	Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
应用	WB, IHC-P, IF, E
稀释方法	IF~~1:100 WB~~1:50~2000 IHC-P~~1:50~1:200
别名	Beclin-1, Coiled-coil myosin-like BCL2-interacting protein, Protein GT197, BECN1, GT197
图像	Fluorescent image of U251 cells stained with APR06985G Beclin1 antibody.U251 cells were treated with Chloroquine (50 μM,16h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with APR06985G Beclin1 primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor? 488 conjugated donkey anti-mouse antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 μg/ml, 5 min). Beclin1 immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells. Western blot analysis of anti-BECN1 Mab (Cat. APR06985G) in HepG2, 293, Jurkat and Hela cell line lysates (35μg/lane). BECN1 (arrow) was detected using the Mab ascites (1:2000 dilution). Western blot analysis of anti-BECN1 Mab (Cat. APR06985G) in recombinant BECN1 protein. BECN1(arrow) was detected using the ascites Mab (1:2000 dilution).
说明书	Product Datasheet Protocol
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