品牌 |
Leading Biology | 货号 |
AMM04366G |
产品分类 |
Monoclonal Antibodies | 研究领域 |
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产品概述 |
We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format.
We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team.
This product is a high quality LC3 antibody (APG8).
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分子量 |
14272 Da
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细胞定位 |
Antigen Cellular Localization:
Cytoplasm, cytoskeleton. Endomembrane system; Lipid-anchor. Cytoplasmic vesicle, autophagosome membrane; Lipid-anchor. Note=LC3-II binds to the autophagic membranes
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宿主 |
Mouse
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种属反应性 |
Human, Mouse, Rat
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靶点 |
This LC3 antibody is generated from mouse immunized with a full length recombinant protein of human LC3 (APG8).
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克隆号 |
166AT1234
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亚型 |
Mouse IgG1 k
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基因ID |
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UniProt ID |
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功能 |
Ubiquitin-like modifier involved in formation of autophagosomal vacuoles (autophagosomes). Whereas LC3s are involved in elongation of the phagophore membrane, the GABARAP/GATE-16 subfamily is essential for a later stage in autophagosome maturation.
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总结 |
MAP1A and MAP1B are microtubule-associated proteins which mediate the physical interactions between microtubules and components of the cytoskeleton. MAP1A and MAP1B each consist of a heavy chain subunit and multiple light chain subunits. The protein encoded by this gene is one of the light chain subunits and can associate with either MAP1A or MAP1B. Two transcript variants encoding different isoforms have been found for this gene.
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形式 |
Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
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储存条件 |
Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
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应用 |
WB, IHC-P, IF, E
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稀释方法 |
WB~~1:1000
IF~~1:200
IHC-P~~1:50~100
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别名 |
Microtubule-associated proteins 1A/1B light chain 3A, Autophagy-related protein LC3 A, Autophagy-related ubiquitin-like modifier LC3 A, MAP1 light chain 3-like protein 1, MAP1A/MAP1B light chain 3 A, MAP1A/MAP1B LC3 A, Microtubule-associated protein 1 light chain 3 alpha, MAP1LC3A
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图像 |
Anti-MAP1LC3A Antibody at 1:5000 dilution + recombinant protein Lysates/proteins at 20 μg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 14 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
All lanes : Anti-MAP1LC3A Antibody at 1:2000 dilution Lane 1: human brain lysates Lane 2: mouse brain lysates Lane 3: rat brain lysates Lysates/proteins at 20 μg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 14 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
Immunofluorescent analysis of U251 cells, using LC3 Antibody (APG8) (Cat. AMM04366G). U251 cells(right) were treated with Chloroquine (50 μM,16h). AMM04366G was diluted at 1:25 dilution. Dylight Fluor 488-conjugated goat anti-mouse lgG at 1:400 dilution was used as the secondary antibody (green). DAPI was used to stain the cell nuclear (blue). |
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说明书 |
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数量 |
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