品牌 |
Leading Biology | 货号 |
APR07707G |
产品分类 |
Monoclonal Antibodies | 研究领域 |
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产品概述 |
We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format.
We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team.
This product is a high quality Enolase-2 antibody gamma (NSE).
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分子量 |
47269 Da
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细胞定位 |
Antigen Cellular Localization:
Cytoplasm. Cell membrane. Note=Can translocate to the plasma membrane in either the homodimeric (alpha/alpha) or heterodimeric (alpha/gamma) form.
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宿主 |
Rabbit
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种属反应性 |
Human, Mouse, Rat
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靶点 |
A synthetic peptide corresponding to residues in human Enolase 2 gamma (NSE) was used as an immunogen.
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克隆号 |
EPR3377
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基因ID |
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UniProt ID |
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功能 |
Has neurotrophic and neuroprotective properties on a broad spectrum of central nervous system (CNS) neurons. Binds, in a calcium-dependent manner, to cultured neocortical neurons and promotes cell survival (By similarity).
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总结 |
Enolase is a glycolytic enzyme that catalyzes the interconversion of 2-phosphoenolpyruvate to phosphoenolpyruvate in the glycolytic pathway (1, 2). The functional enzyme is a dimer made up of subunits referred to as alpha, beta, and gamma. The alpha-, or nonneuronal, enolase (ENO1) is a nearly ubiquitous form, found in almost all tissues, and its expression precedes that of the other isoforms in the early stage of embryonic development. The beta-, or muscle-specific, enolase (ENO3) is present in adult skeletal muscle, and the gamma-, or neuron-specific, enolase (ENO2) is the major form found in mature neurons and in cells of neuronal origin (2).
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形式 |
50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
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储存条件 |
Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
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应用 |
WB, IHC
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稀释方法 |
WB~~1:500
IHC~~1:250~500
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别名 |
Gamma-enolase, 2-phospho-D-glycerate hydro-lyase, Enolase 2, Neural enolase, Neuron-specific enolase, NSE, ENO2
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图像 |
A. Western blot analysis on (A) fetal brain, (B) SH-SY-5Y, (C) HeLa and (D) Y79 cell lysates using anti-Enolase 2 gamma (NSE) RabMAb (Cat. APR07707G), dilution: 1:20,000.
B. Immunohistochemical analysis of paraffin-embedded human brain tissue using anti-Enolase 2 gamme (NSE) RabMAb (Cat. APR07707G). |
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说明书 |
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数量 |
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