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当前位置:首页 > 抗原抗体、ELISA、WB > 一抗 > Monoclonal Antibodies > Creatine Kinase B Antibody

Creatine Kinase B Antibody

货号 货期 规格 / 价格 询价
APG02761G 100 μl / ¥5450

Creatine Kinase B Antibody

品牌

Leading Biology

货号

APG02761G

产品分类

Monoclonal Antibodies

研究领域

产品概述

We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality Creatine Kinase B antibody.

分子量

42644 Da

细胞定位

Antigen Cellular Localization: Cytoplasm.

宿主

Rabbit

种属反应性

Human, Mouse, Rat

靶点

A synthetic peptide corresponding to residues near the C-term of human Creatine Kinase B type was used as immunogen.

克隆号

EPR3927

通用名

CKBB

基因ID

UniProt ID

功能

Reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa.

总结

The creatine kinases are a family of enzymes with a highly conserved protein sequence, which are involved in the maintenance of ATP at sites of cellular work .Three cytoplasmic (non-mitochondrial) isoenzymes of creatine kinase (CK)' are readily identified in human tissue. These isoenzymes are dimeric molecules with two dissociable subunits designated as M- (muscle) or B- (brain) type that can reassociate to form the electrophoretically distinct MM, BB, or MB isotypes (1).The protein encoded by creatine kinase B is a cytoplasmic enzyme involved in energy homeostasis. The encoded protein reversibly catalyzes the transfer of phosphate between ATP and various phosphogens such as creatine phosphate (2). Creatine kinase isoenzymes are crucial to energy metabolism, particularly in tissues with high energy requirements, such as skeletal muscle, heart, brain and spermatozoa (3).

形式

50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

储存条件

Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

应用

WB, IHC, IF

稀释方法

WB~~1:5000~20000 IHC~~1:100~500

别名

Creatine kinase B-type, B-CK, Creatine kinase B chain, CKB, CKBB

图像

Western blot analysis on (A) SHSY-5Y (B) Hela (C) Y-79 (D) SW840 cell lysates using anti-Creatine Kinase B RabMAb (Cat. APG02761G).

Immunohistochemical analysis of paraffin-embedded human glioma tissue using anti-Creatine kinase B chain RabMAb (Cat. APG02761G).

Immunofluorescent staining of HeLa cells using anti-Creatine kinase B chain RabMAb (Cat. APG02761G).

说明书

数量

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