品牌 |
Leading Biology | 货号 |
APG02761G |
产品分类 |
Monoclonal Antibodies | 研究领域 |
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产品概述 |
We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format.
We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team.
This product is a high quality Creatine Kinase B antibody.
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分子量 |
42644 Da
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细胞定位 |
Antigen Cellular Localization:
Cytoplasm.
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宿主 |
Rabbit
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种属反应性 |
Human, Mouse, Rat
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靶点 |
A synthetic peptide corresponding to residues near the C-term of human Creatine Kinase B type was used as immunogen.
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克隆号 |
EPR3927
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通用名 |
CKBB
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基因ID |
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UniProt ID |
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功能 |
Reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa.
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总结 |
The creatine kinases are a family of enzymes with a highly conserved protein sequence, which are involved in the maintenance of ATP at sites of cellular work .Three cytoplasmic (non-mitochondrial) isoenzymes of creatine kinase (CK)' are readily identified in human tissue. These isoenzymes are dimeric molecules with two dissociable subunits designated as M- (muscle) or B- (brain) type that can reassociate to form the electrophoretically distinct MM, BB, or MB isotypes (1).The protein encoded by creatine kinase B is a cytoplasmic enzyme involved in energy homeostasis. The encoded protein reversibly catalyzes the transfer of phosphate between ATP and various phosphogens such as creatine phosphate (2). Creatine kinase isoenzymes are crucial to energy metabolism, particularly in tissues with high energy requirements, such as skeletal muscle, heart, brain and spermatozoa (3).
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形式 |
50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
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储存条件 |
Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
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应用 |
WB, IHC, IF
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稀释方法 |
WB~~1:5000~20000
IHC~~1:100~500
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别名 |
Creatine kinase B-type, B-CK, Creatine kinase B chain, CKB, CKBB
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图像 |
Western blot analysis on (A) SHSY-5Y (B) Hela (C) Y-79 (D) SW840 cell lysates using anti-Creatine Kinase B RabMAb (Cat. APG02761G).
Immunohistochemical analysis of paraffin-embedded human glioma tissue using anti-Creatine kinase B chain RabMAb (Cat. APG02761G).
Immunofluorescent staining of HeLa cells using anti-Creatine kinase B chain RabMAb (Cat. APG02761G). |
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说明书 |
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数量 |
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