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Functional Myosin IIA (non-muscle) (heavy chain) Antibody, mAb (recombinant)

AMP00010G Product Datasheet Protocol

货号	货期	规格 / 价格	询价
AMP00010G		100 μg / ￥5450

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Functional Myosin IIA (non-muscle) (heavy chain) Antibody, mAb (recombinant)

品牌	Leading Biology	货号	AMP00010G
产品分类	Monoclonal Antibodies	研究领域
产品概述	We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality Functional Myosin IIA (non-muscle) (heavy chain) Antibody, mAb (recombinant).
分子量	223044 Da
细胞定位	Antigen Cellular Localization: Cytoplasm, myofibril. Note=Thick filaments of the myofibrils
宿主	Purified From?HEK 293 Cell?culture Supernatant.
种属反应性	Human, Mouse, Rat, Drosophila
靶点	Recognizes human, mouse, rat and drosophila myosin IIA (heavy chain).
克隆号	SF9
亚型	Human IgG2λ
通用名	MYHSA2
基因ID	4620
UniProt ID	Q9UKX2
功能	Muscle contraction. Required for cytoskeleton organization (By similarity).
总结	anti-Myosin IIA (non-muscle), monoclonal antibody (recombinant) (SF9) is an antibody developed by antibody phage display technology using a human naive antibody gene library. These libraries consist of scFv (single chain fragment variable) composed of VH (variable domain of the human immunoglobulin heavy chain) and VL (variable domain of the human immunoglobulin light chain) connected by a polypeptide linker. The antibody fragments are displayed on the surface of filamentous bacteriophage (M13). This scFv was selected by affinity selection on antigen in a process termed panning. Multiple rounds of panning are performed to enrich for antigen-specific scFv-phage. Monoclonal antibodies are subsequently identified by screening after each round of selection. The selected monoclonal scFv is cloned into an appropriate vector containing a Fc portion of interest and then produced in mammalian cells to generate an IgG like scFv-Fc fusion protein.
形式	Liquid. In PBS containing 10% glycerol and 0.02% sodium azide.
储存条件	Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
应用	WB, ICC, E
图像	Human Myosin IIA (non-muscle) (heavy chain) is detected by immunocytochemistry using anti-myosin IIA (non-muscle) (rec.) (SF9). Method:HeLa cells are grown in standard culture conditions, fixed with methanol, and incubated with anti-Myosin IIA (non-muscle) (rec.) (SF9) (1mg /ml in PBS-BSA). After incubation for 30 min at RT and several washes in PBS, cells are treated with a goat anti-human (Cy3) antibody for 30 min at RT, washed and mounted in Moewiol. Nuclei are stained with DAPI. Picture courtesy of Dr. Moutel, Dr. Franck Perez lab, Curie Institute, Paris.
说明书	Product Datasheet Protocol
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