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PPP2R1B Antibody

AMM02520G Product Datasheet Protocol

货号	货期	规格 / 价格	询价
AMM02520G		100 μl / ￥4950

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PPP2R1B Antibody

品牌	Leading Biology	货号	AMM02520G
产品分类	Monoclonal Antibodies	研究领域
产品概述	We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality PPP2R1B Antibody.
分子量	66214 Da
宿主	Mouse
种属反应性	Human, Mouse, Rat
靶点	This PPP2R1B antibody is generated from a mouse immunized with a recombinant protein of human PPP2R1B.
克隆号	1496CT356.164.25.226
亚型	IgG1,Κ
基因ID	5519
UniProt ID	P30154
功能	The PR65 subunit of protein phosphatase 2A serves as a scaffolding molecule to coordinate the assembly of the catalytic subunit and a variable regulatory B subunit.
总结	The PR65 subunit of protein phosphatase 2A serves as a scaffolding molecule to coordinate the assembly of the catalytic subunit and a variable regulatory B subunit.
储存条件	Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
应用	IF, IHC-P, FC, WB, E
稀释方法	IF~~1:25 IHC-P~~1:25 FC~~1:25 WB~~1:4000
图像	Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0. 1% Triton X-100 permeabilized U-2 OS (human bone osteosarcoma cell line) cells labeling Pdx1 with AMM02520G at 1/25 dilution, followed by Dylight? 488-conjugated goat anti-mouse IgG (NA166821) secondary antibody at 1/200 dilution (green). Immunofluorescence image showing cytoplasm staining on U-2 OS cell line. Cytoplasmic actin is detected with Dylight? 554 Phalloidin (PD18466410) at 1/100 dilution (red). The nuclear counter stain is DAPI (blue). AMM02520G staining PPP2R1B in human spleen sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody. Overlay histogram showing Jurkat cells stained with AMM02520G (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AMM02520G, 1:25 dilution) for 60 min at 37?C. The secondary antibody used was Goat-Anti-Mouse IgG, DyLight? 488 Conjugated Highly Cross-Adsorbed(NA168821)) at 1/400 dilution for 40 min at 37?C. Isotype control antibody (blue line) was mouse IgG1 (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
说明书	Product Datasheet Protocol
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