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BRAF Antibody

AMM02330G Product Datasheet Protocol

货号	货期	规格 / 价格	询价
AMM02330G		100 μl / ￥4950

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BRAF Antibody

品牌	Leading Biology	货号	AMM02330G
产品分类	Monoclonal Antibodies	研究领域
产品概述	We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality BRAF Antibody.
分子量	84437 Da
细胞定位	Antigen Cellular Localization: Nucleus. Cytoplasm. Cell membrane. Note=Colocalizes with RGS14 and RAF1 in both the cytoplasm and membranes.
宿主	Mouse
种属反应性	Human
靶点	This BRAF Monoclonal antibody is generated from mouse immunized with BRAF recombinant protein.
克隆号	125CT13.3.5
亚型	IgG1κ
通用名	BRAF1, RAFB1
基因ID	673
UniProt ID	P15056
功能	Protein kinase involved in the transduction of mitogenic signals from the cell membrane to the nucleus. May play a role in the postsynaptic responses of hippocampal neuron. Phosphorylates MAP2K1, and thereby contributes to the MAP kinase signal transduction pathway.
总结	This gene encodes a protein belonging to the raf/mil family of serine/threonine protein kinases. This protein plays a role in regulating the MAP kinase/ERKs signaling pathway, which affects cell division, differentiation, and secretion. Mutations in this gene are associated with cardiofaciocutaneous syndrome, a disease characterized by heart defects, mental retardation and a distinctive facial appearance. Mutations in this gene have also been associated with various cancers, including non-Hodgkin lymphoma, colorectal cancer, malignant melanoma, thyroid carcinoma, non-small cell lung carcinoma, and adenocarcinoma of lung. A pseudogene, which is located on chromosome X, has been identified for this gene.
储存条件	Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
应用	WB, IF, E
稀释方法	WB~~1:500~16000 IF~~1:10~50
图像	All lanes : Anti-BRAF Antibody at 1:1000 dilution Lane 1: MCF-7 whole cell lysate Lane 2: K562 whole cell lysate Lysates/proteins at 20 μg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 84 kDa Blocking/Dilution buffer: 5% NFDM/TBST. Fluorescent image of C2C12 cell stained with BRAF Antibody( AMM02330G).C2C12 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with BRAF primary antibody (1:25, 1 h at 37℃. For secondary antibody, Alexa Fluor? 488 conjugated donkey anti-mouse antibody (green) was used (1:400, 50 min at 37℃.Cytoplasmic actin was counterstained with Alexa Fluor? 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃.BRAF immunoreactivity is localized to Cytoplasm significantly. Western blot analysis of anti-BRAF Antibody in HL-60 cell line lysates (35μg/lane). BRAF (arrow) was detected using the purified Mab.
说明书	Product Datasheet Protocol
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