品牌 |
Swant | 货号 |
3A12 |
产品分类 |
单克隆抗体 | 研究领域 |
Antibodies against neurotransmitters |
产品概述 |
Store stock-solution in freezer (-20°C, not -70°C). Mix stock solution well before making aliquots. 10x diluted solutions may be kept in the refrigerator for some time (several weeks) but should be made 10 mM with sodium azide. Volumes of working dilutions (high dilutions) should be calculated such the they are used up the same day. Avoid polystyrene tubes.
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形式 |
The antibodies have been purified from serum-free culture medium (Tecnomouse bioreactor) by ammoniumsulfate precipitation, dialyzed against water and lyophilized. mAb 3A12 is not suitable for postembedding immunogold procedures at the EM level.
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浓度 |
GABA |
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纯度 |
Mouse monoclonal, IgG1 |
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试剂准备 |
Lyophilized (no preservatives).
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复溶 |
1. Matute C, Streit P, Monoclonal antibodies demonstrating GABA-like immunoreactivity. Histochemistry 1986;86(2):147-57
2. Liu CJ, Grandes P, Matute C, Cuenod M, Streit P: Glutamate-like immunoreactivity revealed in rat olfactory bulb, hippocampus and cerebellum by monoclonal antibody and sensitive staining method. Histochemistry 1989;90(6):427-45
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应用 |
Appropriate final dilutions should be determined experimentally. They may be in the range of 1:10,000 to 1:20,000 or even higher for floating sections, and around 1:1,000 or higher for mounted semithin sections (postembedding staining). These values are for combination with the PAP procedure. If the DAB reaction product in the PAP procedure is silver enhanced (see: Liu et al., Histochem., 1989, 90: 427-445), dilutions in the range of 1:5,000 to 1:20,000 or higher should be adequate for postembedding staining of semithin sections. Add 10% bovine serum to the diluted antibody.
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产品描述 |
Product: Conc. supernat.
Ref: 12
Code No: mAB 3A12
Lot No: ps2
Reconstitution: add the lyophilized material (approx. 1 mg) to 100 ul PBS or another buffered saline (add in order indicated)
allow some time for the material to dissolve; mix by swirling gently
remove particulate matter by centrifugation in a microfuge
add 100 ul glycerol (final glycerol conc. 50% v/v) and mix well (vortex)
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官网链接 |
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数量 |
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选择 | 品牌 | 货号 | 产品名称 | 规格 | 分类 | 研究领域 | 说明书 | 数量 | 目录价 | |
1 | Swant | 2D7 | monoclonal anti-Glutamate antibody | 200 µl | 单克隆抗体 | Antibodies against neurotransmitters |
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¥5096.00 | 订购 询价 | |
2 | Leading Biology | LM21248 | Anti-CD36 Antibody (Rabbit Monoclonal antibody) | 100 ul | 单克隆抗体 | Chondrogenesis Markers |
|
¥4950.00 | 订购 询价 | |
3 | Swant | 288 | Monoclonal antibody to endothelial cells. | 单克隆抗体 |
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¥3243.00 | 订购 询价 | |||
4 | Swant | 345 | Monoclonal antibody to rat pyramidal cells. | 200 µl | 单克隆抗体 | Miscellaneous antibodies |
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¥3243.00 | 订购 询价 | |
5 | Swant | 3D5 | monoclonal anti-GABA antibody | 200 µl | 单克隆抗体 | Antibodies against neurotransmitters |
|
¥5096.00 | 订购 询价 | |
6 | Swant | 77 | mouse monoclonal antibody against rat angiotensinogen | 200 µl | 单克隆抗体 | Antibodies against components of the renin-angiotensin system |
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¥4015.00 | 订购 询价 |
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