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Rabbit anti-Calbindin D-28k

CB-38a (new batch)-200 µl

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Rabbit anti-Calbindin D-28k

品牌	Swant	货号	CB-38a (new batch)-200 µl
产品分类	Antibodies against Ca2+ -bindin	研究领域	Calbindin
产品概述	Make small portions (e.g. 2-5 µl). For long storage, keep at - 80°C (or at least -20°C). For continuous use, keep at 4°C (with 0.01% Na-azide). Avoid repeated freezing and thawing.
形式	This antiserum was produced against recombinant rat calbindin D-28k (CB). It crossreacts with calbindin D-28k from many other species, including human, monkey, rat, mouse chicken and fish (Fig. 1). In immunoblots it recognizes a single band of approximately 27 -28 kDa. In samples with very high (10-fold higher than CB) concentrations of the related protein calretinin, a larger band (29 - 30 kDa) may appear in Western blots. Thus, depending on the dilution of the antiserum and/or sensitivity of the immunostaining, this other calcium-binding protein could be also revealed. In case of doubt, we suggest to switch to Swant’s monoclonal antibody 300 directed against calbindin D-28k, which does not recognize calretinin.
浓度	Calbindin D-28k
纯度	Rabbit
试剂准备	Lyophilized antiserum (no preservatives).
复溶	1. Airaksinen M.S., et al, (1997), PNAS 94(4) : 1488-1493
应用	Immunohistochemistry: 1:10'000 - 1:20'000 with the avidin-biotin method. Immunoblots: 1:100 - 1:1'000. We recommend that the optimal dilutions be determined by titration experiments.
产品描述	Lot No: 9.03 Reconstitution: with 200 µl bidistilled water. Immunoblot: Fig. 1 Immunoblot of brain homogenate of various species with the monoclonal antibody CB 38 1: Mouse; 2: Rat; 3. Guinea pig; 4. Rabbit; 5. Macaca fascicularis; 6. Zebrafish; 7. chicken. In all species, only a band at 28 KDa is detected. Immunohistochemistry on Calbindin D-28k knock-out mice: Antiserum CB38 immunolabels a subpopulation of neurons in the normal brain with high efficiency (Fig. 2a), but does not stain the brain of calretinin knock out mice (Fig. 2b). Product: Antiserum
官网链接	https://www.swant.com/pdfs/Rabbit_anti_calbindin_D-28k_CB38.pdf
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上一产品：Goat antibody to Oncomodulin. 下一产品：Monoclonal anti Calbindin D-28k

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