品牌 |
Swant | 货号 |
7697-200 µl. |
产品分类 |
Antibodies against Ca2+ -bindin | 研究领域 |
Calretinin |
产品概述 |
After reconstitution freeze in small aliquots (e.g. 1 µl) and keep at - 80°C (or at least - 20°C). For continuous use, keep at 4°C (with 0.01% Na-azide). Avoid repeated freezing and thawing.
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形式 |
The antiserum against calretinin is produced in rabbits by immunization with recombinant human calretinin containing a 6-his tag at the N-terminal. The antibody was evaluated for specificity and potency: a) by Biotin-Avidin labeling of cryostate-, vibratome- and paraffinsections of 4% paraformaldehyde fixed brains and b) by immunoenzymatic labelling of immunoblots.
The product is a polyclonal antiserum against calretinin (1), a calcium-binding protein of the EF-hand family related to calbindin D-28k and calmodulin. The antibody reacts specifically with calretinin in tissue originating from human, monkey, rat, mouse, guinea pig, chicken and fish (Fig. 1). This antiserum does not cross-react with calbindin D-28k or other known calcium binding-proteins, as determined by immunoblots and by its distribution in the brain (Fig. 2).
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浓度 |
Calretinin |
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纯度 |
Rabbit |
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试剂准备 |
Lyophilized whole serum (no preservatives).
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复溶 |
1. Schwaller B., Buchwald P., Blümcke I., Celio M.R. and Hunziker W. (1994) Characterization of a polyclonal antiserum against the purified human recombinant calcium-binding protein calretinin. Cell Calcium 14: 639-648.
2. Schiffmann S.N. et al (1999) Impaired motor coordination and Purkinje cell excitability in mice lacking calretinin. PNAS, 96: 5257-5262.
3. Gotzos V., Vogt P. and M.R. Celio (1995) Calretinin is a selective marker for malignant pleural mesotheliomas of the epithelial type. Pathol. Res. Pract. 192:137- 147.
4. Doglioni, C. et al. (1996) Calretinin: a novel immunocytochemical marker for mesothelioma. Am. J. Surg. Pathol. 20:1037-1046.
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应用 |
Immunohistochemistry: 1:2'000 - 1:5'000, on paraformaldehyde (4%) or formalin-fixed tissue, cryostate or paraffin-sections. Immunoblots: 1:1'000 - 1:2'000.
For immunohistochemistry and immunoblots the titer was determined by using the avidin-biotin method. We recommend that the optimal dilutions be determined by titration experiments.
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产品描述 |
Lot No: 1893-0114
Reconstitution: with 200 µl of bidistilled water.
Immunoblot: In Fig. 1, extracts of soluble proteins were isolated from whole brains of different species and separated by SDS-PAGE. In the Western blot the antiserum 7697 specifically recognizes a band of 29-30k Da.Fig. 1 Immunoblot of brain homogenates of various species with antiserum 7697. 1: Mouse, 2: Rat, 3. Guinea pig, 4. Rabbit, 5.Macaca fascicularis, 6. Zebrafish, 7. Chicken, 8. recombinant calretinin
Immunoblot of brain extracts: Antibody 7697 labels a subpopulation of neurons in the normal brain with high efficiency (Fig. 2a), but does not stain the brain of calretinin knock-out mice (Fig. 2b).
Product: Antiserum
uses: Calretinin belong probably to the class of “trigger” calcium binding-proteins. It occurs mainly in subpopulations of nerve cells and is an excellent markers for mesotheliomas(3,4).
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数量 |
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选择 | 品牌 | 货号 | 产品名称 | 规格 | 分类 | 研究领域 | 说明书 | 数量 | 目录价 | |
1 | Swant | 300pur-100 µl | Affinity purified mouse monoclonal anti Calbindin D-28k | 100 µl | Antibodies against Ca2+ -bindin | Calbindin |
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¥5096.00 | 订购 询价 | |
2 | Swant | PVG213-200 ul. | Goat anti-parvalbumin | 200 ul. | Antibodies against Ca2+ -bindin | Parvalbumin |
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¥5945.00 | 订购 询价 | |
3 | Swant | PV27-200 µl | Rabbit anti-parvalbumin | 200 µl | Antibodies against Ca2+ -bindin | Parvalbumin |
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¥6022.00 | 订购 询价 | |
4 | Swant | OMG4-200 µl. | Goat antibody to Oncomodulin. | 200 µl. | Antibodies against Ca2+ -bindin | Others |
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¥4246.00 | 订购 询价 | |
5 | Swant | 8181-200 µl. | Rabbit anti-Hpcal4 (hippocalcin-like 4) | 200 µl. | Antibodies against Ca2+ -bindin | Others |
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¥4324.00 | 订购 询价 | |
6 | Swant | CG1-200 µl. | Goat antibody to Calretinin. | 200 µl. | Antibodies against Ca2+ -bindin | Calretinin |
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¥5096.00 | 订购 询价 |
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