您好,欢迎光临安诺伦(北京)生物科技商城!
全国服务热线:400-965-8633
关注我们
  • 会员中心
  • 会员中心
    询价列表
    0

    最新加入的商品

    0 件商品 合计:¥ 0
  • 会员中心
    购物车

    最新加入的商品

    件商品 合计:¥ 0

全部产品分类

自主品牌
当前位置:首页 > 抗原抗体、ELISA、WB > 一抗 > Antibodies against Ca2+ -bindin > Monoclonal anti Calbindin D-28k
品牌:
  • 所有品牌
  • A
  • B
  • C
  • D
  • E
  • F
  • G
  • H
  • I
  • J
  • K
  • L
  • M
  • N
  • O
  • P
  • Q
  • R
  • S
  • T
  • U
  • V
  • W
  • X
  • Z

Monoclonal anti Calbindin D-28k

300-1 ml
货号 货期 规格 / 价格 询价

Monoclonal anti Calbindin D-28k

品牌

Swant

货号

300-1 ml

产品分类

Antibodies against Ca2+ -bindin

研究领域

Calbindin

产品概述

1. Celio M.R. et al., Cell Calcium 11:599-602, 1990 2. Kretsinger R.H. (1981) Neurosci. Res. Progr. Bull. 19/8, MIT-Press 3. Garcia-Segura L.M. et al. (1984) Brain Rews. 296: 75-86. 4. Airaksinen M.S., et al, (1997), PNAS 94(4) : 1488-1493

形式

Monoclonal anti-calbindin D-28k is a mouse IgG1 produced by hybridization of mouse myeloma cells with spleen cells from mice immunized with calbindin D-28k purified from chicken gut (1). The antibody was evaluated for specificity and potency: a) by indirect immunofluorescent or immunoperoxidase labelling as well as Biotin-Avidin labeling of cryostate or vibratome-sections of 4 % paraformaldehyde fixed tissue; b) by immunoenzymatic labelling of immunoblots; c) by radioimmunoassay. The product is a monoclonal antibody (McAB) against calbindin D-28k, a calcium-binding protein of the EF-hand family related to calmodulin and troponin-C. McAB 300 reacts specifically with calbindin D-28k on immunoblots of extracts of tissue originating from human, monkey, guinea pig, rabbit, rat, mouse and chicken (but probably not fish, Fig. 1). McAB 300 does not cross-react with calretinin or other known calcium binding-proteins. McAB 300 specifically stains the 45Ca-binding spot of calbindin D-28k (MW 28'000, IEP 4.8) in a two-dimensional gel. In radioimmunoassay it detects calbindin D-28k with a sensitivity of 10 ng/assay and an affinity of 1.6 x 1012 L/M. McAB 300 against calbindin D-28k specifically localizes calbindin using free-floating or mounted sections of brain, kidney and pancreas of probably all vertebrates with the exception of fishes.

浓度

Calbindin D-28k

纯度

Mouse monoclonal, IgG1

试剂准备

Lyophilized concentrated supernatant (no preservatives).

复溶

1. Celio M.R. et al., Cell Calcium 11:599-602, 1990 2. Kretsinger R.H. (1981) Neurosci. Res. Progr. Bull. 19/8, MIT-Press 3. Garcia-Segura L.M. et al. (1984) Brain Rews. 296: 75-86. 4. Airaksinen M.S., et al, (1997), PNAS 94(4) : 1488-1493

应用

Immunohistochemistry: 1:5'000 - 1:10'000 with the avidin-biotin method. Immunoblots: 1:100 - 1:1'000 with an indirect peroxidase method. We recommend that the optimal dilutions be determined by titration experiments.

产品描述

Lot No: 07 (F) Reconstitution: with 200 µl / 500 µl or 1 ml bidistilled water. Immunoblot of brain extracts: Fig. 1 Immunoblot of brain homogenate of various species with the monoclonal antibody CB300 1: Mouse; 2: Rat; 3. Guinea pig; 4. Rabbit; 5. Macaca fascicularis; 6. Zebrafish; 7. recombinant calbindin D- 28k. In all species, only a band at 28 KDa is detected. Immunohistochemistry on Calbindin D-28k knock-out mice: Fig. 1 Immunoblot of brain homogenate of various species with the monoclonal antibody CB300 1: Mouse; 2: Rat; 3. Guinea pig; 4. Rabbit; 5. Macaca fascicularis; 6. Zebrafish; 7. recombinant calbindin D- 28k. In all species, only a band at 28 KDa is detected. Product: Conc. supernat.

官网链接

数量

相关产品

选择 品牌 货号 产品名称 规格 分类 研究领域 说明书 数量 目录价
1 Swant 300pur-100 µl Affinity purified mouse monoclonal anti Calbindin D-28k 100 µl Antibodies against Ca2+ -bindin Calbindin
¥5096.00 订购 询价
2 Swant PVG213-200 ul. Goat anti-parvalbumin 200 ul. Antibodies against Ca2+ -bindin Parvalbumin
¥5945.00 订购 询价
3 Swant PV27-200 µl Rabbit anti-parvalbumin 200 µl Antibodies against Ca2+ -bindin Parvalbumin
¥6022.00 订购 询价
4 Swant OMG4-200 µl. Goat antibody to Oncomodulin. 200 µl. Antibodies against Ca2+ -bindin Others
¥4246.00 订购 询价
5 Swant 8181-200 µl. Rabbit anti-Hpcal4 (hippocalcin-like 4) 200 µl. Antibodies against Ca2+ -bindin Others
¥4324.00 订购 询价
6 Swant CG1-200 µl. Goat antibody to Calretinin. 200 µl. Antibodies against Ca2+ -bindin Calretinin
¥5096.00 订购 询价

产品添加成功!

购物车已有 0 件产品

加入询价单成功!

询价单已有 0 件产品