品牌 |
Swant | 货号 |
PV27-200 µl |
产品分类 |
Antibodies against Ca2+ -bindin | 研究领域 |
Parvalbumin |
产品概述 |
Reconstitute with 200 µl bi-distilled water and make small portions upon arrival (e.g. 2-5 µl). For long storage, keep at - 80°C (or at least - 20°C). For continuous use keep the diluted antiserum at 4°C (with 0.01% Na-azide). Avoid repeated freezing and thawing.
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形式 |
This antiserum was produced against recombinant rat parvalbumin. It cross-reacts with many other species, humans included. It can be used in immunohistochemistry (Fig. 1) and for immunoblotting (Fig. 2).
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浓度 |
Parvalbumin |
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纯度 |
Rabbit |
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试剂准备 |
Lyophilized antiserum (no preservatives).
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复溶 |
1. Kretsinger R.H. (1981) Neurosci. Res. Progr. Bull. 19/8, MIT-Press
2. Celio M.R., Heizmann C.W. (1981) Nature 293: 300-302
3. Celio M.R., Heizmann C.W. (1982) Nature 297:504-506
4. Schwaller B., et al. (1999) Am. J. Physiol. 276. C395-403
5. *Filice F, Celio M.R., Szabolcsi V. (2017) JCN, in press
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应用 |
Immunohistochemistry: 1:5'000 - 1:10'000 with the avidin-biotin method. Immunoblots: 1:500 - 1:1'000 We recommend that the optimal dilutions be determined by titration experiments.
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产品描述 |
Lot No: 2014
Reconstitution: with 200 µl / 500 µl / 1 ml bi-distilled water (depending on the volume you ordered).
Immunoblot: The antiserum PV27 recognizes the antigen at 12 kDa after SDS-gel electrophoretic separation of brain extracts. Therefore, antiserum PV27 can be used in immunoblots (Fig. 2).
Immunohistochemistry on Calbindin D-28k knock-out mice: Antiserum PV27 labels a subpopulation of neurons in the normal brain with high efficiency (Fig. 1a), but does not stain the brain of parvalbumin knock-out mice (Fig. 1b).
Product: Antiserum
Background: Calcium binding-proteins represent a family of small, acidic proteins equipped with peculiar cavities which accept Ca2+ with high selectivity (1). There are two types of calcium binding-proteins, “trigger” and “buffer” proteins. Those of the “trigger”-type (e.g. calmodulin and troponin-C) act by changing shape upon binding Ca2+. This distortion exposes regions on the surface of the protein, which interact with surrounding target molecules, altering their activity. The calcium binding-protein of the “buffer”-type are conceived as a system which is in charge of controlling the Ca2+ concentration inside cells. Parvalbumin occurs mainly in subpopulations of nerve cells (2) and in fast muscle fibers (3). It might confer on these cells peculiar skills in the handling of calcium-ions.
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数量 |
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选择 | 品牌 | 货号 | 产品名称 | 规格 | 分类 | 研究领域 | 说明书 | 数量 | 目录价 | |
1 | Swant | 300pur-100 µl | Affinity purified mouse monoclonal anti Calbindin D-28k | 100 µl | Antibodies against Ca2+ -bindin | Calbindin |
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¥5096.00 | 订购 询价 | |
2 | Swant | PVG213-200 ul. | Goat anti-parvalbumin | 200 ul. | Antibodies against Ca2+ -bindin | Parvalbumin |
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¥5945.00 | 订购 询价 | |
3 | Swant | OMG4-200 µl. | Goat antibody to Oncomodulin. | 200 µl. | Antibodies against Ca2+ -bindin | Others |
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¥4246.00 | 订购 询价 | |
4 | Swant | 8181-200 µl. | Rabbit anti-Hpcal4 (hippocalcin-like 4) | 200 µl. | Antibodies against Ca2+ -bindin | Others |
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¥4324.00 | 订购 询价 | |
5 | Swant | CG1-200 µl. | Goat antibody to Calretinin. | 200 µl. | Antibodies against Ca2+ -bindin | Calretinin |
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¥5096.00 | 订购 询价 | |
6 | Swant | GP72-100 µl. | Guinea pig antiserum against parvalbumin | 100 µl. | Antibodies against Ca2+ -bindin | Parvalbumin |
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¥5018.00 | 订购 询价 |
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