品牌 |
Swant | 货号 |
300-200 µl |
产品分类 |
Antibodies against Ca2+ -bindin | 研究领域 |
Calbindin |
产品概述 |
For long storage, keep at - 80°C (or at least - 20°C). For continuous use, keep at 4°C (with 0.01% Na-azide). Avoid repeated freezing and thawing.
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形式 |
Monoclonal anti-calbindin D-28k is a mouse IgG1 produced by hybridization of mouse myeloma cells with spleen cells from mice immunized with calbindin D-28k purified from chicken gut (1). The antibody was evaluated for specificity and potency: a) by indirect immunofluorescent or immunoperoxidase labelling as well as Biotin-Avidin labeling of cryostate or vibratome-sections of 4 % paraformaldehyde fixed tissue; b) by immunoenzymatic labelling of immunoblots; c) by radioimmunoassay.
The product is a monoclonal antibody (McAB) against calbindin D-28k, a calcium-binding protein of the EF-hand family related to calmodulin and troponin-C.
McAB 300 reacts specifically with calbindin D-28k on immunoblots of extracts of tissue originating from human, monkey, guinea pig, rabbit, rat, mouse and chicken (but probably not fish, Fig. 1). McAB 300 does not cross-react with calretinin or other known calcium binding-proteins. McAB 300 specifically stains the 45Ca-binding spot of calbindin D-28k (MW 28'000, IEP 4.8) in a two-dimensional gel. In radioimmunoassay it detects calbindin D-28k with a sensitivity of 10 ng/assay and an affinity of 1.6 x 1012 L/M. McAB 300 against calbindin D-28k specifically localizes calbindin using free-floating or mounted sections of brain, kidney and pancreas of probably all vertebrates with the exception of fishes.
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浓度 |
Calbindin D-28k |
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纯度 |
Mouse monoclonal, IgG1 |
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试剂准备 |
Lyophilized concentrated supernatant (no preservatives).
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复溶 |
1. Celio M.R. et al., Cell Calcium 11:599-602, 1990
2. Kretsinger R.H. (1981) Neurosci. Res. Progr. Bull. 19/8, MIT-Press
3. Garcia-Segura L.M. et al. (1984) Brain Rews. 296: 75-86.
4. Airaksinen M.S., et al, (1997), PNAS 94(4) : 1488-1493
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应用 |
Immunohistochemistry: 1:5'000 - 1:10'000 with the avidin-biotin method.
Immunoblots: 1:100 - 1:1'000 with an indirect peroxidase method.
We recommend that the optimal dilutions be determined by titration experiments.
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产品描述 |
Lot No: 07 (F)
Reconstitution: with 200 µl / 500 µl or 1 ml bidistilled water.
Immunoblot of brain extracts: Fig. 1 Immunoblot of brain homogenate of various species with the monoclonal antibody CB300 1: Mouse; 2: Rat; 3. Guinea pig; 4. Rabbit; 5. Macaca fascicularis; 6. Zebrafish; 7. recombinant calbindin D- 28k. In all species, only a band at 28 KDa is detected.
Immunohistochemistry on Calbindin D-28k knock-out mice: Antibody CB300 immunolabels a subpopulation of neurons in the normal brain with high efficiency (Fig. 2a) but does not stain in the brain of calbindin D-28k knock out mice (Fig. 2).
Product: Conc. supernat.
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官网链接 |
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数量 |
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选择 | 品牌 | 货号 | 产品名称 | 规格 | 分类 | 研究领域 | 说明书 | 数量 | 目录价 | |
1 | Swant | 300pur-100 µl | Affinity purified mouse monoclonal anti Calbindin D-28k | 100 µl | Antibodies against Ca2+ -bindin | Calbindin |
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¥5096.00 | 订购 询价 | |
2 | Swant | PVG213-200 ul. | Goat anti-parvalbumin | 200 ul. | Antibodies against Ca2+ -bindin | Parvalbumin |
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¥5945.00 | 订购 询价 | |
3 | Swant | PV27-200 µl | Rabbit anti-parvalbumin | 200 µl | Antibodies against Ca2+ -bindin | Parvalbumin |
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¥6022.00 | 订购 询价 | |
4 | Swant | OMG4-200 µl. | Goat antibody to Oncomodulin. | 200 µl. | Antibodies against Ca2+ -bindin | Others |
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¥4246.00 | 订购 询价 | |
5 | Swant | 8181-200 µl. | Rabbit anti-Hpcal4 (hippocalcin-like 4) | 200 µl. | Antibodies against Ca2+ -bindin | Others |
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¥4324.00 | 订购 询价 | |
6 | Swant | CG1-200 µl. | Goat antibody to Calretinin. | 200 µl. | Antibodies against Ca2+ -bindin | Calretinin |
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¥5096.00 | 订购 询价 |
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