品牌 |
CELLSCRIPT | 货号 |
C-PAP5104H |
产品分类 |
RNA转录试剂盒 | 研究领域 |
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产品概述 |
Store at -20°C in a freezer without a defrost cycle. Do not store at -70°C
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形式 |
The A-Plus™ Poly(A) Polymerase Tailing Kit uses ATP as a substrate for template-independent additionof adenosine monophosphates to the 3'-hydroxyl termini of RNA. The standard prot°Col produces apoly(A)-tail length of ~150 b on 40-60 μg of RNA.Polyadenylation increases the stability of RNA ineukaryotic cells and enhances its ability to be translated after transfection or microinjection.1-3 A Poly(A)tail is useful to provide a priming site for first-strand cDNA synthesis in certain applications, and can beused to end-label4 or quantify5 mRNA.
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产品描述 |
Unit Definition
One unit of A-Plus Poly(A) Polymerase converts 1 nmole of ATP into acid-insoluble material in 10minutes at 37°C under standard assay reaction conditions.
Functional Testing
The A-Plus Poly(A) Polymerase Tailing Kit is functionally tested in 1X A-Plus Poly(A) Tailing Bufferwith 1 mM ATP and a 1.4 kb transcript.
Contaminating Activity Assays
All components of the A-Plus Poly(A) Polymerase Tailing Kit are free of detectable RNase and DNaseactivity.
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官网链接 |
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数量 |
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选择 | 品牌 | 货号 | 产品名称 | 规格 | 分类 | 研究领域 | 说明书 | 数量 | 目录价 | |
1 | CELLSCRIPT | C-MSC11610 | T7 mScript™ Standard mRNA Production System | 10 reactions | RNA转录试剂盒 |
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¥6600.00 | 订购 询价 | ||
2 | CELLSCRIPT | C-SCCS1710 | ScriptCap™ Cap 1 Capping System | 10 reactions | RNA转录试剂盒 |
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¥4169.00 | 订购 询价 | ||
3 | CELLSCRIPT | C-CC15011H | Cap-Clip™ Acid Pyrophosphatase | 100 units | RNA转录试剂盒 |
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¥10625.00 | 订购 询价 | ||
4 | CELLSCRIPT | C-SRI6310K | ScriptGuard™ RNase Inhibitor | 10,000 units | RNA转录试剂盒 |
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¥5865.00 | 订购 询价 | ||
5 | CELLSCRIPT | C-ASF3507 | T7-FlashScribe™ Transcription Kit | 50 reactions | RNA转录试剂盒 |
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¥6005.00 | 订购 询价 | ||
6 | CELLSCRIPT | C-ICTMY110510 | INCOGNITO™ T7 5mC- & -RNA Transcription Kit | 10 reactions | RNA转录试剂盒 |
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¥13093.00 | 订购 询价 |
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