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T7-FlashScribe™ Transcription Kit

C-ASF3507

货号	货期	规格 / 价格	询价
C-ASF3507		50 reactions / ￥5005.00

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T7-FlashScribe™ Transcription Kit

品牌	CELLSCRIPT	货号	C-ASF3507
产品分类	RNA转录试剂盒	研究领域
产品概述	Store at -20°C in a freezer without a defrost cycle. Do not store at -70°C
形式	The T7-FlashScribe™ Transcription Kit is specially formulated to enable users to obtain the maximumpossible yields of RNA from an in vitro transcription (IVT) reaction in just 30 minutes. The standard30 minute, 20 μl reaction will yield up to 180 μg of RNA from 1 μg of the control template. These yieldsare made possible by the high-performance properties of the T7-FlashScribe enzyme. The T7-FlashScribe Transcription Kit produces exceptionally high yields of either long or short transcripts.The standard reaction can be scaled up to produce milligram amounts of RNA. T7-FlashScribe IVT RNA can be pr°Cessed into mRNA (5'-end capped and 3'-end poly(A) tailed) throughthe use of CELLSCRIPT's ScriptCap™ m7G Capping System, ScriptCap 2'-O-Methyltransferase Kit andA-Plus™ Poly(A) Polymerase Tailing Kit (available separately). CELLSCRIPT also offers the INCOGNITO™ line of transcription kits for the production of pseudouridine-& 5-methyl-cytosine-containing (GA C and GA 5mC) IVT RNA. It has been shown that -mRNAs and5mC-mRNAs are translated into protein at higher levels and induce lower innate immune responses inhuman and other mammalian cells that express various RNA sensors compared t°Correspondingunmodified mRNAs.1-4
试剂准备	RNase-Free DNase I is provided in a 50% glycerol solution containing 50 mM Tris-HCl, pH 7.5,10 mM CaCl2, 10 mM MgCl2 and 0.1% Triton® X-100. ScriptGuard RNase Inhibitor is supplied in a50% glycerol solution containing 50 mM Tris-HCl, pH 7.5, 100 mM NaCl, 10 mM DTT, 0.1 mM EDTAand 0.1% Triton® X-100. All other enzymes are provided in a 50% glycerol solution containing 50 mMTris-HCl, pH 7.5, 100 mM NaCl, 1 mM DTT, 0.1 mM EDTA and 0.1% Triton X-100.
产品描述	Unit Definitions One unit of RNase-Free DNase I digests 1 µg of pUC19 DNA to oligodeoxynucleotides in 10 minutesat 37°C. One unit of ScriptGuard RNase Inhibitor results in 50% inhibition of 5 ng of RNase A. Activity ismeasured by the inhibition of hydrolysis of cyclic 2',3'-CMP by RNase A. Functional Testing The T7-FlashScribe Transcription Kit is functionally tested under standard reaction conditions usingthe T7 Control Template DNA. The kit must produce at least 136 µg of RNA from 1 µg of the T7Control Template DNA in 30 minutes at 37°C. Contaminating Activity Assays All components of the T7-FlashScribe Transcription Kit are free of detectable RNase and DNaseactivity, except for the inherent activity of the RNase-Free DNase I component.
官网链接	http://www.cellscript.com/products/018pl0617CS.pdf
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上一产品：AmpliCap-Max™ T7 High Yield Message Maker Kit 下一产品：T7 mScript™ Standard mRNA Production System

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