Noradrenaline Transporter (NET) (extracellular) Antibody

We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality Noradrenaline Transporter (NET) (extracellular) antibody.

69255 Da

Rabbit

Mouse, Rat

Peptide?? (C)KLLNASVLGDHTKYSK, corresponding to amino acid residues 189-204 of mouse Noradrenaline Transporter (Accession O55192). 2nd extracellular loop.

Many physiological, endocrine and behavioral functions are determined and regulated by monoamine signaling1,2. Many brain disorders such as depression, drug abuse, schizophrenia, attention deficit hyperactivity disorder (ADHD) are caused by the malfunction of monoaminergic transmission1-3. The intensity of monoaminergic signaling is determined bythe availability of the monoamine,which is in turn determined in part by its uptake from the extracellular milieu via monoamine transporters. These transporters include DAT, SERT, and NET, responsible for uptaking dopamine, serotonin and noradrenaline respectively, and recycling them back for release3-5.While the activity of each transporter is faithful to its neurotransmitter, NET has been shown to clear dopamine in DAT deprived or low DAT regions such as the brain cortex6-8.DAT, SERT and NET are members of the Na+/Cl- dependent membrane transporter family which also includes other members. These transporters consist of 12 transmembrane domains and intracellular N- and C-termini. NET also has a significant extracellular loop between transmembrane regions three and four, which contains various glycosylation sites9. Like its counterparts, NET’s intracellular N- and C-terminal domains are also subject to phosphorylation and protein-protein interactions important for modulating its activity and localization. In addition alternative splicing has also been shown to regulate NET’s expression and function10.NET is specifically expressed on noradrenaline nerve terminals, and is also expressed in the periphery, such as adrenal glands and placenta. NET malfunction is largely associated with attention and mood, as well as various cardiovascular disorders. NET is also a target for psychostimulants such as cocaine and amphetamines which disrupt its function, thereby causing an increase of noradrenaline in synaptic clefts3,9.Abgent is pleased to offer a highly specific antibody directed against an extracellular epitope of mouse noradrenaline transporter. Anti-Noradrenaline Transporter (NET) (extracellular) antibody (#AG1206) can be used for western blot immunohistochemistry and immunocytochemistry applications. It has been designed to recognize NET from human, mouse and rat samples.

Affinity purified antibody, Liquid

Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

WB

WB~~1:200-1:2000 IHC~~1:200 ICC~~1:100

Sodium-dependent noradrenaline transporter, Norepinephrine transporter, NET, Solute carrier family 6 member 2, Slc6a2

Product Datasheet Protocol