品牌 |
Swant | 货号 |
rCB28 |
产品分类 |
Recombinant Protein | 研究领域 |
Calbindin |
产品概述 |
After reconstitution, freeze in small aliquots (e.g. 1 ug) and keep at -80°C. Avoid repeated freezing and thawing.
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形式 |
This recombinant protein has been purified by FPLC and migrates as a single band. The figure shows a gel stained with a very sensitive silver stain.
Silver stained SDS-PAGE of FPLCpurified rat recombinant calbindinD-28k. The arrow mark the full lenghth native calbindin D-28k (28 kDa). This preparation may contain trace amounts of Escherichia Coli proteins
This protein can be used for immunoblots, absorption experiments in immunohistochemistry, radioimmunoassay and intracellular injection.
For adsorption we suggest the following procedure:
- Dilute 1 µl of the monoclonal antibody 300 against calbindin D-28k in 5 ml of the usual buffer for immunohistochemistry (final dilution 1:5'000).
- Add 1 µg of the recombinant protein to 1 ml of the diluted antibody solution and mix well.
- Incubate for at least 6 hours in the cold.
- Apply to tissue-sections and incubate for 3 days.
- Complete the immunohistochemical reaction as usual (biotinylated second antibody, ABC-complex, DAB).
As a result, the immunostaining should be strongly reduced or even completely prevented.
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浓度 |
Rat calbindin D-28k (recombinant) produced in Escherichia Coli. |
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纯度 |
Recombinant rat |
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试剂准备 |
lyophilized 10 or 100 µg in 12 µl of 50 mM NaHCo3.
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产品描述 |
Purification: HPLC
Lot No: 1 (17.07.06)
Reconstitution: with 10 or 20 µl of bidistilled water.
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官网链接 |
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数量 |
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选择 | 品牌 | 货号 | 产品名称 | 规格 | 分类 | 研究领域 | 说明书 | 数量 | 目录价 | |
1 | Swant | rCB9 | Calbindin D-9k | 60 µg | Recombinant Protein | Calbindin |
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¥8488.00 | 订购 询价 | |
2 | Swant | AgPV100ic | Parvalbumin | 100 µg | Recombinant Protein | Parvalbumin |
|
¥8138.00 | 订购 询价 | |
3 | Swant | AgPV10abs | Parvalbumin | 10 µg | Recombinant Protein | Parvalbumin |
|
¥3150.00 | 订购 询价 | |
4 | Swant | rCRh100 | Calretinin | 100 µg | Recombinant Protein | Calretinin |
|
¥12688.00 | 订购 询价 | |
5 | Swant | AgCB10ic | Calbindin D-28k | 10 µg | Recombinant Protein | Calbindin |
|
¥3150.00 | 订购 询价 | |
6 | Swant | AgPV10ic | Parvalbumin | 10 µg | Recombinant Protein | Parvalbumin |
|
¥3150.00 | 订购 询价 |
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